KL 1604 then U2 7927 at Schiphol
Rome → Amsterdam → Prague
Timetable, Amsterdam time
15:20KL 1604 from Rome at the gate
13:10U2 7927 to Prague leaves the gate
21 h 50 apart on the timetable, overnight
U2 7927 leaves 21 h 50 after KL 1604 arrives on the timetable: more than half a day, so not a connection we count.