KL 644 then U2 7925 at Schiphol
New York JFK → Amsterdam → Prague
Timetable, Amsterdam time
10:30KL 644 from New York JFK at the gate
07:00U2 7925 to Prague leaves the gate
20 h 30 apart on the timetable, overnight
U2 7925 leaves 20 h 30 after KL 644 arrives on the timetable: more than half a day, so not a connection we count.