A3 275 then GQ 254 at Athens
Chios → Athens → Souda
Timetable, Athens time
17:00A3 275 from Chios at the gate
16:30GQ 254 to Souda leaves the gate
23 h 30 apart on the timetable, overnight
GQ 254 leaves 23 h 30 after A3 275 arrives on the timetable: more than half a day, so not a connection we count.