EW 2522 then EW 7525 at Barcelona
Stuttgart → Barcelona → Hamburg
Timetable, Barcelona time
20:00EW 2522 from Stuttgart at the gate
19:00EW 7525 to Hamburg leaves the gate
23 h apart on the timetable, overnight
EW 7525 leaves 23 h after EW 2522 arrives on the timetable: more than half a day, so not a connection we count.