EW 7520 then FR 6341 at Barcelona
Hamburg → Barcelona → Rome
Timetable, Barcelona time
16:20EW 7520 from Hamburg at the gate
05:45FR 6341 to Rome leaves the gate
13 h 25 apart on the timetable, overnight
FR 6341 leaves 13 h 25 after EW 7520 arrives on the timetable: more than half a day, so not a connection we count.