EW 7520 then FR 6927 at Barcelona
Hamburg → Barcelona → Rome
Timetable, Barcelona time
16:20EW 7520 from Hamburg at the gate
16:00FR 6927 to Rome leaves the gate
23 h 40 apart on the timetable, overnight
FR 6927 leaves 23 h 40 after EW 7520 arrives on the timetable: more than half a day, so not a connection we count.