EW 7520 then VY 6012 at Barcelona
Hamburg → Barcelona → Rome
Timetable, Barcelona time
16:20EW 7520 from Hamburg at the gate
15:40VY 6012 to Rome leaves the gate
23 h 20 apart on the timetable, overnight
VY 6012 leaves 23 h 20 after EW 7520 arrives on the timetable: more than half a day, so not a connection we count.