FR 3181 then TK 1854 at Barcelona
Rome → Barcelona → Istanbul
Timetable, Barcelona time
15:05FR 3181 from Rome at the gate
12:20TK 1854 to Istanbul leaves the gate
21 h 15 apart on the timetable, overnight
TK 1854 leaves 21 h 15 after FR 3181 arrives on the timetable: more than half a day, so not a connection we count.