AA 4521 then DL 5626 at Boston
Rochester → Boston → Charlotte
Timetable, Boston time
06:30AA 4521 from Rochester at the gate
06:20DL 5626 to Charlotte leaves the gate
23 h 50 apart on the timetable, overnight
DL 5626 leaves 23 h 50 after AA 4521 arrives on the timetable: more than half a day, so not a connection we count.