AA 4570 then AA 359 at Boston
Washington National → Boston → Charlotte
Timetable, Boston time
14:42AA 4570 from Washington National at the gate
14:39AA 359 to Charlotte leaves the gate
23 h 57 apart on the timetable, overnight
AA 359 leaves 23 h 57 after AA 4570 arrives on the timetable: more than half a day, so not a connection we count.