AA 1004 then AA 5377 at Charlotte
Boston → Charlotte → Rochester
Timetable, Charlotte time
09:44AA 1004 from Boston at the gate
09:08AA 5377 to Rochester leaves the gate
23 h 24 apart on the timetable, overnight
AA 5377 leaves 23 h 24 after AA 1004 arrives on the timetable: more than half a day, so not a connection we count.