AA 1094 then AA 5543 at Charlotte
Fort Myers → Charlotte → Pittsburgh
Timetable, Charlotte time
16:50AA 1094 from Fort Myers at the gate
08:35AA 5543 to Pittsburgh leaves the gate
15 h 45 apart on the timetable, overnight
AA 5543 leaves 15 h 45 after AA 1094 arrives on the timetable: more than half a day, so not a connection we count.