AA 1485 then AA 5093 at Charlotte
Rochester → Charlotte → Sarasota
Timetable, Charlotte time
09:23AA 1485 from Rochester at the gate
09:20AA 5093 to Sarasota leaves the gate
23 h 57 apart on the timetable, overnight
AA 5093 leaves 23 h 57 after AA 1485 arrives on the timetable: more than half a day, so not a connection we count.