AA 1576 then AA 473 at Charlotte
Jacksonville → Charlotte → Washington
Timetable, Charlotte time
08:59AA 1576 from Jacksonville at the gate
06:00AA 473 to Washington leaves the gate
21 h 01 apart on the timetable, overnight
AA 473 leaves 21 h 01 after AA 1576 arrives on the timetable: more than half a day, so not a connection we count.