AA 1576 then AA 5262 at Charlotte
Jacksonville → Charlotte → Lexington
Timetable, Charlotte time
08:59AA 1576 from Jacksonville at the gate
08:35AA 5262 to Lexington leaves the gate
23 h 36 apart on the timetable, overnight
AA 5262 leaves 23 h 36 after AA 1576 arrives on the timetable: more than half a day, so not a connection we count.