AA 1576 then AA 5641 at Charlotte
Jacksonville → Charlotte → Charleston (South Carolina)
Timetable, Charlotte time
08:59AA 1576 from Jacksonville at the gate
07:14AA 5641 to Charleston (South Carolina) leaves the gate
22 h 15 apart on the timetable, overnight
AA 5641 leaves 22 h 15 after AA 1576 arrives on the timetable: more than half a day, so not a connection we count.