AA 1617 then AA 5262 at Charlotte
Washington National → Charlotte → Lexington
Timetable, Charlotte time
07:50AA 1617 from Washington National at the gate
07:17AA 5262 to Lexington leaves the gate
23 h 27 apart on the timetable, overnight
AA 5262 leaves 23 h 27 after AA 1617 arrives on the timetable: more than half a day, so not a connection we count.