AA 2189 then AA 5935 at Charlotte
Madison → Charlotte → Charlottesville
Timetable, Charlotte time
21:47AA 2189 from Madison at the gate
21:45AA 5935 to Charlottesville leaves the gate
23 h 58 apart on the timetable, overnight
AA 5935 leaves 23 h 58 after AA 2189 arrives on the timetable: more than half a day, so not a connection we count.