AA 2320 then AA 5364 at Charlotte
Rochester → Charlotte → Melbourne (Florida)
Timetable, Charlotte time
20:56AA 2320 from Rochester at the gate
20:50AA 5364 to Melbourne (Florida) leaves the gate
23 h 54 apart on the timetable, overnight
AA 5364 leaves 23 h 54 after AA 2320 arrives on the timetable: more than half a day, so not a connection we count.