AA 2437 then AA 3221 at Charlotte
Pittsburgh → Charlotte → Tampa
Timetable, Charlotte time
09:35AA 2437 from Pittsburgh at the gate
07:28AA 3221 to Tampa leaves the gate
21 h 53 apart on the timetable, overnight
AA 3221 leaves 21 h 53 after AA 2437 arrives on the timetable: more than half a day, so not a connection we count.