AA 2477 then AA 5543 at Charlotte
Orlando → Charlotte → Pittsburgh
Timetable, Charlotte time
14:25AA 2477 from Orlando at the gate
08:35AA 5543 to Pittsburgh leaves the gate
18 h 10 apart on the timetable, overnight
AA 5543 leaves 18 h 10 after AA 2477 arrives on the timetable: more than half a day, so not a connection we count.