AA 2486 then AA 5543 at Charlotte
St Louis → Charlotte → Pittsburgh
Timetable, Charlotte time
16:49AA 2486 from St Louis at the gate
08:35AA 5543 to Pittsburgh leaves the gate
15 h 46 apart on the timetable, overnight
AA 5543 leaves 15 h 46 after AA 2486 arrives on the timetable: more than half a day, so not a connection we count.