AA 2538 then AA 5565 at Charlotte
Tampa → Charlotte → Richmond
Timetable, Charlotte time
08:53AA 2538 from Tampa at the gate
08:24AA 5565 to Richmond leaves the gate
23 h 31 apart on the timetable, overnight
AA 5565 leaves 23 h 31 after AA 2538 arrives on the timetable: more than half a day, so not a connection we count.