AA 2538 then UA 3302 at Charlotte
Tampa → Charlotte → Rochester
Timetable, Charlotte time
08:53AA 2538 from Tampa at the gate
07:30UA 3302 to Rochester leaves the gate
22 h 37 apart on the timetable, overnight
UA 3302 leaves 22 h 37 after AA 2538 arrives on the timetable: more than half a day, so not a connection we count.