AA 2544 then AA 5318 at Charlotte
Columbus (Ohio) → Charlotte → Lexington
Timetable, Charlotte time
20:19AA 2544 from Columbus (Ohio) at the gate
19:28AA 5318 to Lexington leaves the gate
23 h 09 apart on the timetable, overnight
AA 5318 leaves 23 h 09 after AA 2544 arrives on the timetable: more than half a day, so not a connection we count.