AA 2544 then AA 5395 at Charlotte
Columbus (Ohio) → Charlotte → Daytona Beach
Timetable, Charlotte time
20:17AA 2544 from Columbus (Ohio) at the gate
19:50AA 5395 to Daytona Beach leaves the gate
23 h 33 apart on the timetable, overnight
AA 5395 leaves 23 h 33 after AA 2544 arrives on the timetable: more than half a day, so not a connection we count.