AA 2544 then AA 6063 at Charlotte
Columbus (Ohio) → Charlotte → Gainesville
Timetable, Charlotte time
20:19AA 2544 from Columbus (Ohio) at the gate
19:48AA 6063 to Gainesville leaves the gate
23 h 29 apart on the timetable, overnight
AA 6063 leaves 23 h 29 after AA 2544 arrives on the timetable: more than half a day, so not a connection we count.