AA 2555 then AA 5543 at Charlotte
Fort Myers → Charlotte → Pittsburgh
Timetable, Charlotte time
13:10AA 2555 from Fort Myers at the gate
08:35AA 5543 to Pittsburgh leaves the gate
19 h 25 apart on the timetable, overnight
AA 5543 leaves 19 h 25 after AA 2555 arrives on the timetable: more than half a day, so not a connection we count.