AA 2680 then AA 5543 at Charlotte
New York JFK → Charlotte → Pittsburgh
Timetable, Charlotte time
14:34AA 2680 from New York JFK at the gate
08:35AA 5543 to Pittsburgh leaves the gate
18 h 01 apart on the timetable, overnight
AA 5543 leaves 18 h 01 after AA 2680 arrives on the timetable: more than half a day, so not a connection we count.