AA 2700 then AA 5543 at Charlotte
Boston → Charlotte → Pittsburgh
Timetable, Charlotte time
12:00AA 2700 from Boston at the gate
08:35AA 5543 to Pittsburgh leaves the gate
20 h 35 apart on the timetable, overnight
AA 5543 leaves 20 h 35 after AA 2700 arrives on the timetable: more than half a day, so not a connection we count.