AA 2854 then AA 5435 at Charlotte
Louisville → Charlotte → Dayton
Timetable, Charlotte time
19:45AA 2854 from Louisville at the gate
19:16AA 5435 to Dayton leaves the gate
23 h 31 apart on the timetable, overnight
AA 5435 leaves 23 h 31 after AA 2854 arrives on the timetable: more than half a day, so not a connection we count.