AA 301 then AA 5543 at Charlotte
Grand Cayman → Charlotte → Pittsburgh
Timetable, Charlotte time
15:10AA 301 from Grand Cayman at the gate
08:30AA 5543 to Pittsburgh leaves the gate
17 h 20 apart on the timetable, overnight
AA 5543 leaves 17 h 20 after AA 301 arrives on the timetable: more than half a day, so not a connection we count.