AA 3129 then AA 5318 at Charlotte
Jacksonville → Charlotte → Lexington
Timetable, Charlotte time
19:45AA 3129 from Jacksonville at the gate
19:28AA 5318 to Lexington leaves the gate
23 h 43 apart on the timetable, overnight
AA 5318 leaves 23 h 43 after AA 3129 arrives on the timetable: more than half a day, so not a connection we count.