AA 3129 then AA 5406 at Charlotte
Jacksonville → Charlotte → Knoxville
Timetable, Charlotte time
19:45AA 3129 from Jacksonville at the gate
19:30AA 5406 to Knoxville leaves the gate
23 h 45 apart on the timetable, overnight
AA 5406 leaves 23 h 45 after AA 3129 arrives on the timetable: more than half a day, so not a connection we count.