AA 3129 then AA 5869 at Charlotte
Jacksonville → Charlotte → Tri-Cities
Timetable, Charlotte time
19:45AA 3129 from Jacksonville at the gate
19:35AA 5869 to Tri-Cities leaves the gate
23 h 50 apart on the timetable, overnight
AA 5869 leaves 23 h 50 after AA 3129 arrives on the timetable: more than half a day, so not a connection we count.