AA 3253 then AA 5310 at Charlotte
Indianapolis → Charlotte → Cincinnati
Timetable, Charlotte time
12:53AA 3253 from Indianapolis at the gate
12:52AA 5310 to Cincinnati leaves the gate
23 h 59 apart on the timetable, overnight
AA 5310 leaves 23 h 59 after AA 3253 arrives on the timetable: more than half a day, so not a connection we count.