AA 380 then AA 5543 at Charlotte
New York LaGuardia → Charlotte → Pittsburgh
Timetable, Charlotte time
14:19AA 380 from New York LaGuardia at the gate
08:35AA 5543 to Pittsburgh leaves the gate
18 h 16 apart on the timetable, overnight
AA 5543 leaves 18 h 16 after AA 380 arrives on the timetable: more than half a day, so not a connection we count.