AA 387 then AA 5262 at Charlotte
Detroit → Charlotte → Lexington
Timetable, Charlotte time
08:06AA 387 from Detroit at the gate
07:17AA 5262 to Lexington leaves the gate
23 h 11 apart on the timetable, overnight
AA 5262 leaves 23 h 11 after AA 387 arrives on the timetable: more than half a day, so not a connection we count.