AA 5019 then AA 5533 at Charlotte
Lexington → Charlotte → Gulfport
Timetable, Charlotte time
21:44AA 5019 from Lexington at the gate
21:35AA 5533 to Gulfport leaves the gate
23 h 51 apart on the timetable, overnight
AA 5533 leaves 23 h 51 after AA 5019 arrives on the timetable: more than half a day, so not a connection we count.