AA 5035 then AA 5262 at Charlotte
Manchester → Charlotte → Lexington
Timetable, Charlotte time
08:02AA 5035 from Manchester at the gate
07:17AA 5262 to Lexington leaves the gate
23 h 15 apart on the timetable, overnight
AA 5262 leaves 23 h 15 after AA 5035 arrives on the timetable: more than half a day, so not a connection we count.