AA 5075 then AA 5222 at Charlotte
Roanoke → Charlotte → Washington
Timetable, Charlotte time
17:29AA 5075 from Roanoke at the gate
17:15AA 5222 to Washington leaves the gate
23 h 46 apart on the timetable, overnight
AA 5222 leaves 23 h 46 after AA 5075 arrives on the timetable: more than half a day, so not a connection we count.