AA 5169 then AA 5984 at Charlotte
Detroit → Charlotte → Florence
Timetable, Charlotte time
13:10AA 5169 from Detroit at the gate
13:05AA 5984 to Florence leaves the gate
23 h 55 apart on the timetable, overnight
AA 5984 leaves 23 h 55 after AA 5169 arrives on the timetable: more than half a day, so not a connection we count.