AA 5185 then AA 5543 at Charlotte
Columbus (Ohio) → Charlotte → Pittsburgh
Timetable, Charlotte time
16:04AA 5185 from Columbus (Ohio) at the gate
08:35AA 5543 to Pittsburgh leaves the gate
16 h 31 apart on the timetable, overnight
AA 5543 leaves 16 h 31 after AA 5185 arrives on the timetable: more than half a day, so not a connection we count.