AA 5191 then AA 6009 at Charlotte
Knoxville → Charlotte → Tallahassee
Timetable, Charlotte time
07:29AA 5191 from Knoxville at the gate
07:26AA 6009 to Tallahassee leaves the gate
23 h 57 apart on the timetable, overnight
AA 6009 leaves 23 h 57 after AA 5191 arrives on the timetable: more than half a day, so not a connection we count.