AA 5220 then AA 5588 at Charlotte
Washington → Charlotte → Asheville
Timetable, Charlotte time
14:57AA 5220 from Washington at the gate
14:39AA 5588 to Asheville leaves the gate
23 h 42 apart on the timetable, overnight
AA 5588 leaves 23 h 42 after AA 5220 arrives on the timetable: more than half a day, so not a connection we count.