AA 5231 then AA 5475 at Charlotte
Charleston (West Virginia) → Charlotte → Lexington
Timetable, Charlotte time
07:41AA 5231 from Charleston (West Virginia) at the gate
07:22AA 5475 to Lexington leaves the gate
23 h 41 apart on the timetable, overnight
AA 5475 leaves 23 h 41 after AA 5231 arrives on the timetable: more than half a day, so not a connection we count.