AA 5241 then AA 5133 at Charlotte
Melbourne (Florida) → Charlotte → Louisville
Timetable, Charlotte time
14:39AA 5241 from Melbourne (Florida) at the gate
14:20AA 5133 to Louisville leaves the gate
23 h 41 apart on the timetable, overnight
AA 5133 leaves 23 h 41 after AA 5241 arrives on the timetable: more than half a day, so not a connection we count.