AA 5241 then AA 6112 at Charlotte
Melbourne (Florida) → Charlotte → Knoxville
Timetable, Charlotte time
14:39AA 5241 from Melbourne (Florida) at the gate
14:06AA 6112 to Knoxville leaves the gate
23 h 27 apart on the timetable, overnight
AA 6112 leaves 23 h 27 after AA 5241 arrives on the timetable: more than half a day, so not a connection we count.