AA 5258 then AA 2562 at Charlotte
Lexington → Charlotte → St Louis
Timetable, Charlotte time
20:55AA 5258 from Lexington at the gate
20:52AA 2562 to St Louis leaves the gate
23 h 57 apart on the timetable, overnight
AA 2562 leaves 23 h 57 after AA 5258 arrives on the timetable: more than half a day, so not a connection we count.