AA 5281 then AA 5227 at Charlotte
Cincinnati → Charlotte → Birmingham (Alabama)
Timetable, Charlotte time
10:15AA 5281 from Cincinnati at the gate
23:00AA 5227 to Birmingham (Alabama) leaves the gate
12 h 45 apart on the timetable
AA 5227 leaves 12 h 45 after AA 5281 arrives on the timetable: more than half a day, so not a connection we count.